Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17729] to p53 (phospho T55)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-p53 (phospho T55) antibody [EPR17729]
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EPR17729] to p53 (phospho T55)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Whole cell lysate of HEK-293 cells treated with 200nM Calyculin A, 60J/m2 UV and/or 1uM Okadaic Acid for 60 minutes.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)
    Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)
    All lanes : Anti-p53 (phospho T55) antibody [EPR17729] (ab183546) at 1/1000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate membrane incubated with phosphatase
    Lane 3 : Whole cell lysate from HEK-293 (Human epithelial cell line from embryonic kidney) cells, treated with UV at 60J/m2 for 2 hours

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)
    Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)
    All lanes : Anti-p53 (phospho T55) antibody [EPR17729] (ab183546) at 1/1000 dilution

    Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 minutes.
    Lane 2 : Untreated HEK-293 whole cell lysate.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time of 15 sec

  • Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)
    Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546) This image is courtesy of an Abreview submitted by Christian Marx

    Western blot analysis of HCT116 p53wt and HCT116 p53-/- cells were treated with DMSO (Control) or 15/24h with 500 nM doxorubicin (ab120629) labelling p53 (phospho T55) with ab183546 at 1/2500. A HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody (1/5000). GAPDH (ab128915) was used as protein loading control.

    See Abreview

  • Dot Blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)
    Dot Blot - Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)

    Dot blot analysis of p53 (phospho T55) using ab183546 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

    Lane 1: Phospho peptide

    Lane 2: Non-phospho peptide

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time of 3 minutes.

  • Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)
    Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-p53 (phospho T55) antibody [EPR17729] (ab183546)

© 2021 Astana Biomed Group LLP. All rights reserved.