Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LSECtin antibody [EPR13724] (ab181196)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13724] to LSECtin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-LSECtin antibody [EPR13724]
    See all LSECtin primary antibodies

  • Description

    Rabbit monoclonal [EPR13724] to LSECtin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver lysate and mouse liver lysates. IHC-P: Human lymph node lysate and tissue, Human liver tissue.

  • General notes

    Previously labelled as CLEC4G.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)

    Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-LSECtin antibody [EPR13724] (ab181196)
    Western blot - Anti-LSECtin antibody [EPR13724] (ab181196)
    Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution + Mouse liver lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-LSECtin antibody [EPR13724] (ab181196)
    Western blot - Anti-LSECtin antibody [EPR13724] (ab181196)
    All lanes : Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human lymph node lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 33 kDa

  • Anti-LSECtin antibody [EPR13724] (ab181196)
    Anti-LSECtin antibody [EPR13724] (ab181196)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.