Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Wednesday March 03, 2021

Anti-LSECtin antibody [EPR13724] (ab181196)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13724] to LSECtin
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-LSECtin antibody [EPR13724]
See all LSECtin primary antibodies
Description

Rabbit monoclonal [EPR13724] to LSECtin
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver lysate and mouse liver lysates. IHC-P: Human lymph node lysate and tissue, Human liver tissue.
General notes
Previously labelled as CLEC4G.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13724
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-LSECtin antibody [EPR13724] (ab181196)

Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution + Mouse liver lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Exposure time: 180 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-LSECtin antibody [EPR13724] (ab181196)

All lanes : Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human lymph node lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 33 kDa
Anti-LSECtin antibody [EPR13724] (ab181196)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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