Anti-NOP58 antibody [EPR10721] (ab155969)
![Anti-NOP58 antibody [EPR10721] (ab155969)](https://www.abcam.com/ps/products/155/ab155969/Images/ab155969-163267-anti-nop58-antibody-epr10721-western-blot.jpg "Anti-NOP58 antibody [EPR10721] (ab155969)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10721] to NOP58
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-NOP58 antibody [EPR10721]
See all NOP58 primary antibodies -
Description
Rabbit monoclonal [EPR10721] to NOP58 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide, corresponding to residues in Human NOP58 (Uniprot: Q9Y2X3).
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Positive control
- HeLa, K562, MCF7 and NCI-H460 cell lysates; Human hepatocellular carcinoma tissue; MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10721 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NOP58 antibody [EPR10721] (ab155969)All lanes : Anti-NOP58 antibody [EPR10721] (ab155969) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : NCI-H450 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 60 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody [EPR10721] (ab155969)](https://www.abcam.com/ps/products/155/ab155969/Images/ab155969-163266-anti-nop58-antibody-epr10721-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody [EPR10721] (ab155969)Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue, labeling NOP58 with ab155969 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-NOP58 antibody [EPR10721] (ab155969)](https://www.abcam.com/ps/products/155/ab155969/Images/ab155969-163265-anti-nop58-antibody-epr10721-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NOP58 antibody [EPR10721] (ab155969)Immunofluorescence analysis of MCF7 cells, labeling NOP58 with ab155969 at 1/250 dilution. -
![Anti-NOP58 antibody [EPR10721] (ab155969)](https://www.abcam.com/ps/products/155/ab155969/Images/ab155969-4-benefits-of-recombinant-antibodies.png)
Anti-NOP58 antibody [EPR10721] (ab155969)
