Anti-Vitamin B2 antibody [EPR20853-141] (ab218125)
![Anti-Vitamin B2 antibody [EPR20853-141] (ab218125)](https://www.abcam.com/ps/products/218/ab218125/Images/ab218125-1-218125Dot1.jpg "Anti-Vitamin B2 antibody [EPR20853-141] (ab218125)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20853-141] to Vitamin B2
- Suitable for: Dot blot
Overview
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Product name
Anti-Vitamin B2 antibody [EPR20853-141]
See all Vitamin B2 primary antibodies -
Description
Rabbit monoclonal [EPR20853-141] to Vitamin B2 -
Host species
Rabbit -
Tested applications
Suitable for: Dot blotmore details -
Immunogen
Chemical/ Small Molecule corresponding to Vitamin B2 conjugated to bovine serum albumin.
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Positive control
- Dot blot: Ovalbumin (OVA)-Riboflavin.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20853-141 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-Vitamin B2 antibody [EPR20853-141] (ab218125)
Dot blot analysis of Vitamin B12 using ab218125 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Lane 1: Ovalbumin (OVA)-Riboflavin
Lane 2: OVA-Folic acid (carboxyl conjugation)
Lane 3: OVA-Folic acid (amino conjugation)
Lane 4: OVA-Histamine
Exposure time: 3 minutes.
Blocking/Diluting buffer: 5% NFDM/TBST
Folic acid and Histamine are the analogue of Riboflavin.
Ovalbumin (OVA)-conjugated small molecules are kindly provided by Dr Rao Yi.
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![Anti-Vitamin B2 antibody [EPR20853-141] (ab218125)](https://www.abcam.com/ps/products/218/ab218125/Images/ab218125-2-benefits-of-recombinant-antibodies.png)
Anti-Vitamin B2 antibody [EPR20853-141] (ab218125)
