Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20131] to Prothrombin - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Prothrombin antibody [EPR20131] - BSA and Azide free
    See all Prothrombin primary antibodies

  • Description

    Rabbit monoclonal [EPR20131] to Prothrombin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251497 is the carrier-free version of ab208590.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20131

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)

    This data was developed using ab208590, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Prothrombin with ab208590 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse liver was observed (PMID: 1705822). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)

    This data was developed using ab208590, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Prothrombin with ab208590 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on plasma and endothelial cells of human kidney was observed (PMID: 16410745). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)
    Western blot - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)
    All lanes : Anti-Prothrombin antibody [EPR20131] (ab208590) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 70 kDa
    Observed band size: 72 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab208590, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)
    Western blot - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)
    All lanes : Anti-Prothrombin antibody [EPR20131] (ab208590) at 1/1000 dilution

    Lane 1 : Mouse plasma
    Lane 2 : Rat plasma

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 70 kDa
    Observed band size: 33,48,72 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab208590, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Prothrombin is proteolytically cleaved into different fragments. The fragment profile is consistent with the literature (PMID: 17637839; 21131592; 16734589).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)

    This data was developed using ab208590, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Prothrombin with ab208590 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat liver was observed (PMID: 1705822). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)
    Anti-Prothrombin antibody [EPR20131] - BSA and Azide free (ab251497)

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