Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19765] to Fibrinogen beta chain - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free
    See all Fibrinogen beta chain primary antibodies

  • Description

    Rabbit monoclonal [EPR19765] to Fibrinogen beta chain - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human Fibrinogen beta chain.
    Database link: P02675

  • General notes

    ab251177 is the carrier-free version of ab193932.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
    Immunocytochemistry - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
    This data was developed using ab193932, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Fibrinogen beta chain with ab193932 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining of Fibrinogen beta on HepG2 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
    Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
    All lanes : Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932) at 1/2000 dilution

    Lane 1 : His-tagged human fibrinogen alpha chain recombinant protein (aa401-866)
    Lane 2 : GST-His-tagged human fibrinogen beta chain recombinant protein (aa1-491)
    Lane 3 : GST-His-tagged human fibrinogen gamma chain recombinant protein (aa1-453)

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab193932, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
    Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
    All lanes : Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human plasma
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    This data was developed using ab193932, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2: 1 second; Lane 3: 3 minutes.

  • Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
    Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)

    This data was developed using ab193932, the same antibody clone in a different buffer formulation.

    Fibrinogen beta chain was immunoprecipitated from 0.35 mg of HepG2 (human liver hepatocellular carcinoma cell line) lysate with ab193932 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab193932 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HepG2 whole cell lysate 10 µg (Input).

    Lane 2: ab193932 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193932 in HepG2 whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
    Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)

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