Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)
![Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)](https://www.abcam.com/ps/products/251/ab251496/Images/ab251496-406416-anti-prothrombin-antibody-epr20159-bsa-and-azide-free-western-blot.jpg "Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20159] to Prothrombin - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Overview
-
Product name
Anti-Prothrombin antibody [EPR20159] - BSA and Azide free
See all Prothrombin primary antibodies -
Description
Rabbit monoclonal [EPR20159] to Prothrombin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab251496 is the carrier-free version of ab208589.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20159 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)All lanes : Anti-Prothrombin antibody [EPR20159] (ab208589) at 1/1000 dilution
Lane 1 : Human plasma at 20 µg
Lane 2 : Human fetal liver tissue lysate at 20 µg
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 70 kDa
Observed band size: 33,52,72 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab208589, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Prothrombin is proteolytically cleaved into different fragments. The fragments profile is consistent with what has been described in the literature (PMID: 17637839; 21131592; 16734589).
-
![Immunocytochemistry - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)](https://www.abcam.com/ps/products/251/ab251496/Images/ab251496-2-anti-prothrombin-antibody-epr20159-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)This data was developed using ab208589, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Prothrombin with ab208589 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. -
![Flow Cytometry - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)](https://www.abcam.com/ps/products/251/ab251496/Images/ab251496-1-anti-prothrombin-antibody-epr20159-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)This data was developed using ab208589, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Prothrombin with ab208589 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. -
![Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)](https://www.abcam.com/ps/products/251/ab251496/Images/ab251496-4-benefits-of-recombinant-antibodies.png)
Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)
