Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20159] to Prothrombin - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Prothrombin antibody [EPR20159] - BSA and Azide free
    See all Prothrombin primary antibodies

  • Description

    Rabbit monoclonal [EPR20159] to Prothrombin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251496 is the carrier-free version of ab208589.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20159

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)
    Western blot - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)
    All lanes : Anti-Prothrombin antibody [EPR20159] (ab208589) at 1/1000 dilution

    Lane 1 : Human plasma at 20 µg
    Lane 2 : Human fetal liver tissue lysate at 20 µg
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 70 kDa
    Observed band size: 33,52,72 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab208589, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Prothrombin is proteolytically cleaved into different fragments. The fragments profile is consistent with what has been described in the literature (PMID: 17637839; 21131592; 16734589).

  • Immunocytochemistry - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)
    Immunocytochemistry - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)
    This data was developed using ab208589, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Prothrombin with ab208589 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Flow Cytometry - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)
    Flow Cytometry - Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)
    This data was developed using ab208589, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Prothrombin with ab208589 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
  • Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)
    Anti-Prothrombin antibody [EPR20159] - BSA and Azide free (ab251496)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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