Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)
![Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)](https://www.abcam.com/ps/products/76/ab76107/Images/ab76107-91436-anti-cytochrome-c-antibody-ep1326-80-5-immunohistochemistry.gif "Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1326-80-5] to Cytochrome C
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, African green monkey
Overview
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Product name
Anti-Cytochrome C antibody [EP1326-80-5]
See all Cytochrome C primary antibodies -
Description
Rabbit monoclonal [EP1326-80-5] to Cytochrome C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Cytochrome C (N terminal). The exact sequence is proprietary.
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Positive control
- HeLa, COS and L929 cell lysates; human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1326-80-5 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)
ab76107 at 1/100 dilution staining Cytochrome C in human kidney by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)](https://www.abcam.com/ps/products/76/ab76107/Images/ab76107-91435-anti-cytochrome-c-antibody-ep1326-80-5-western-blot.gif)
Western blot - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)All lanes : Anti-Cytochrome C antibody [EP1326-80-5] (ab76107) at 1/200 dilution
Lane 1 : HeLa cell lysate
Lane 2 : COS cell lysate
Lane 3 : L929 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat anti-rabbit HRP at 1/2000 dilution
Lane 3 : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 12 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
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![Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)](https://www.abcam.com/ps/products/76/ab76107/Images/ab76107-3-benefits-of-recombinant-antibodies.png)
Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)
