Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Tuesday March 09, 2021

Anti-POR1 antibody [EPR11943] (ab180503)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11943] to POR1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Reacts with: Human

Product name

Anti-POR1 antibody [EPR11943]
See all POR1 primary antibodies
Description

Rabbit monoclonal [EPR11943] to POR1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- SH-SY5Y, MCF-7, HeLa and HepG2 whole cell lysate (ab7900); Human gastric carcinoma tissue; SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11943
Isotype

IgG
Research areas

Western blot - Anti-POR1 antibody [EPR11943] (ab180503)

All lanes : Anti-POR1 antibody [EPR11943] (ab180503) at 1/5000 dilution

Lane 1 : SH-SY5Y cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 38 kDa
Immunoprecipitation - Anti-POR1 antibody [EPR11943] (ab180503)

Western blot analysis of SH-SY5Y cell lysate (Lane 1) and Negative control (Lane 2) immunoprecipitated with ab180503 at 1/30 dilution. Rabbit TrueBlot®:Anti-Rabbit IgG HRP secondary antibody used at 1/1500 dilution.
Flow Cytometry - Anti-POR1 antibody [EPR11943] (ab180503)

Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling POR2 with ab180503 at 1/150 dilution (red), compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-POR1 antibody [EPR11943] (ab180503)

Immunofluorescent analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling POR2 with ab180503 at 1/1000 dilution, followed by Goat anti rabbit IgG (Dylight 555) secondary antibody at 1/250 dilution. Counter stained with DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POR1 antibody [EPR11943] (ab180503)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling POR2 with ab180503 at 1/500 dilution, followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-POR1 antibody [EPR11943] (ab180503)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com