Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-POR1 antibody [EPR11943] (ab180503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11943] to POR1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-POR1 antibody [EPR11943]
    See all POR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11943] to POR1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SH-SY5Y, MCF-7, HeLa and HepG2 whole cell lysate (ab7900); Human gastric carcinoma tissue; SH-SY5Y cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11943

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-POR1 antibody [EPR11943] (ab180503)
    Western blot - Anti-POR1 antibody [EPR11943] (ab180503)
    All lanes : Anti-POR1 antibody [EPR11943] (ab180503) at 1/5000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa

  • Immunoprecipitation - Anti-POR1 antibody [EPR11943] (ab180503)
    Immunoprecipitation - Anti-POR1 antibody [EPR11943] (ab180503)

    Western blot analysis of SH-SY5Y cell lysate (Lane 1) and Negative control (Lane 2) immunoprecipitated with ab180503 at 1/30 dilution. Rabbit TrueBlot®:Anti-Rabbit IgG HRP secondary antibody used at 1/1500 dilution.

  • Flow Cytometry - Anti-POR1 antibody [EPR11943] (ab180503)
    Flow Cytometry - Anti-POR1 antibody [EPR11943] (ab180503)

    Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling POR2 with ab180503 at 1/150 dilution (red), compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-POR1 antibody [EPR11943] (ab180503)
    Immunocytochemistry/ Immunofluorescence - Anti-POR1 antibody [EPR11943] (ab180503)

    Immunofluorescent analysis of  4% paraformaldehyde-fixed SH-SY5Y cells labeling POR2 with ab180503 at 1/1000 dilution, followed by Goat anti rabbit IgG (Dylight 555) secondary antibody at 1/250 dilution. Counter stained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POR1 antibody [EPR11943] (ab180503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POR1 antibody [EPR11943] (ab180503)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling POR2 with ab180503 at 1/500 dilution, followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-POR1 antibody [EPR11943] (ab180503)
    Anti-POR1 antibody [EPR11943] (ab180503)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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