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Anti-CD94 antibody [EPR21003] - BSA and Azide free (ab238166)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21003] to CD94 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD94 antibody [EPR21003] - BSA and Azide free
    See all CD94 primary antibodies

  • Description

    Rabbit monoclonal [EPR21003] to CD94 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human NK/T-cell lymphoma tissue.

  • General notes

    Ab238166 is the carrier-free version of ab235441. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238166 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21003

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-CD94 antibody [EPR21003] - BSA and Azide free (ab238166)
    Immunoprecipitation - Anti-CD94 antibody [EPR21003] - BSA and Azide free (ab238166)

    CD94 was immunoprecipitated from 0.35 mg NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate with ab235441 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235441 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.


    Lane 1: NK-92 whole cell lysate 10 µg (Input).
    Lane 2: ab235441 IP in NK-92 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235441 in NK-92 whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 10358137; PMID: 8969479). The diffuse bands between 20-30 kDa are likely due to glycosylation.

    This blot was developed with a high sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235441).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD94 antibody [EPR21003] - BSA and Azide free (ab238166)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD94 antibody [EPR21003] - BSA and Azide free (ab238166)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD94 with ab235441 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in the marginal zone of human tonsil (PMID: 11253136) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235441).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD94 antibody [EPR21003] - BSA and Azide free (ab238166)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD94 antibody [EPR21003] - BSA and Azide free (ab238166)

    Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue labeling CD94 with ab235441 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human NK/T-cell lymphoma (PMID: 11253136, PMID: 10828054, PMID: 12816864) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab235441).

     

  • Anti-CD94 antibody [EPR21003] - BSA and Azide free (ab238166)
    Anti-CD94 antibody [EPR21003] - BSA and Azide free (ab238166)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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