Anti-B7-H6 antibody [EPR21841] - BSA and Azide free (ab237035)
![Anti-B7-H6 antibody [EPR21841] - BSA and Azide free (ab237035)](https://www.abcam.com/ps/products/237/ab237035/Images/ab237035-314429-anti-b7-h6-antibody-epr21841-western-blot.jpg "Anti-B7-H6 antibody [EPR21841] - BSA and Azide free (ab237035)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21841] to B7-H6 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-B7-H6 antibody [EPR21841] - BSA and Azide free
See all B7-H6 primary antibodies -
Description
Rabbit monoclonal [EPR21841] to B7-H6 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, Raji and HeLa whole cell lysates.
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General notes
Ab237035 is the carrier-free version of ab219205. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab237035 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21841 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-B7-H6 antibody [EPR21841] - BSA and Azide free (ab237035)All lanes : Anti-B7-H6 antibody [EPR21841] (ab219205) at 1/1000 dilution
Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 whole cell lysate treated with PNGase F
Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDaExposure time : Lanes 1 and 2: 92 seconds; Lanes 3 and 4: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
B7-H6 is a glycoprotein and can be de-glycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID 24780758).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219205).
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![Anti-B7-H6 antibody [EPR21841] - BSA and Azide free (ab237035)](https://www.abcam.com/ps/products/237/ab237035/Images/ab237035-2-benefits-of-recombinant-antibodies.png)
Anti-B7-H6 antibody [EPR21841] - BSA and Azide free (ab237035)
