Anti-B7-H6 antibody (ab229999)
Key features and details
- Rabbit polyclonal to B7-H6
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-B7-H6 antibody
See all B7-H6 primary antibodies -
Description
Rabbit polyclonal to B7-H6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Orangutan
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Immunogen
Recombinant fragment corresponding to Human B7-H6 aa 1-300.
Database link: Q68D85 -
Positive control
- WB: Mouse heart, liver and brain tissue lysates; HeLa whole cell lysate (ab150035). IHC-P: Human melanoma tissue. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-B7-H6 antibody (ab229999)All lanes : Anti-B7-H6 antibody (ab229999) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 32,38,51 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-B7-H6 antibody (ab229999)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for B7-H6 (green) using ab229999 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L) as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7-H6 antibody (ab229999)Paraffin-embedded human melanoma tissue stained for B7-H6 using ab229999 at 1/100 dilution in immunohistochemical analysis.
