Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20257] to Myeloperoxidase
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Myeloperoxidase antibody [EPR20257]
See all Myeloperoxidase primary antibodies
Description

Rabbit monoclonal [EPR20257] to Myeloperoxidase
Host species

Rabbit
Specificity

This antibody is specific to Myeloperoxidase heavy chain.

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal spleen lysate; Rat and mouse spleen lysates; HL-60 whole cell lysate. IHC-P: Human spleen and stomach cancer tissues; Mouse and rat spleen tissues. ICC/IF: HL-60 cells. Flow Cyt: Mouse PBMC and HL-60 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20257
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

All lanes : Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/1000 dilution

Lane 1 : Human fetal spleen lysate
Lane 2 : Rat spleen lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 59 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID: 3029127 PMID: 2154223.

Negative control: HeLa PMID 12040446.
Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunofluorescent analysis of 100% methanol-fixed HL-60 (Human promyelocytic leukemia cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Myeloperoxidase with ab208670 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on HL-60 cell line.

Negative control: HeLa (PMID: 12040446).

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of human stomach cancer is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa cells (left panel) and HL-60 cells (right panel) labeling Myeloperoxidase with ab208670 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control ((ab172730); black) and unlabelled control (cells without incubation with primary and secondary antibodies; blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control: HeLa (PMID: 12040446).
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/5000 dilution + Mouse spleen lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID: 3029127 PMID: 2154223.
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/20000 dilution + HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 39,59,89 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID: 2154223 PMID: 8384653.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of mouse spleen is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of rat spleen is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Flow cytometric analysis of 4% paraformaldehyde-fixed Mouse PBMC cells labeling Myeloperoxidase with ab208670 at 1/500 dilution (right), compared with a rabbit monoclonal IgG isotype control (ab172730; left). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Mouse peripheral blood mononuclear cells stained intracellularly with ab208670 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.
Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

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