Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9473] to Arg2
Suitable for: ICC/IF, Flow Cyt, WB
Reacts with: Human

Product name

Anti-Arg2 antibody [EPR9473]
See all Arg2 primary antibodies
Description

Rabbit monoclonal [EPR9473] to Arg2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Arg2 aa 300-400 (C terminal). The exact sequence is proprietary.
Database link: P78540
Positive control
- Caco 2, Jurkat and 293T cell lysates; Caco 2 cells; JAR whole cell lysates; HEK-293.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9473
Isotype

IgG
Research areas

Western blot - Anti-Arg2 antibody [EPR9473] (ab137069)

Anti-Arg2 antibody [EPR9473] (ab137069) at 1/20000 dilution (purified) + Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 39 kDa

Blocking and diluting buffer : 5% NFDM/TBST
Flow Cytometry - Anti-Arg2 antibody [EPR9473] (ab137069)

Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Arg2 with purified ab137069 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-Arg2 antibody [EPR9473] (ab137069)

Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Arg2 with Purified ab137069 at 1:500 dilution (1.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Arg2 antibody [EPR9473] (ab137069)

Anti-Arg2 antibody [EPR9473] (ab137069) at 1/10000 dilution (purified) + JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 39 kDa

Blocking and diluting buffer : 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Arg2 antibody [EPR9473] (ab137069)

Immunofluorescent analysis of Caco 2 cells labelling Arg2 with ab137069 at 1/250 dilution.
Flow Cytometry - Anti-Arg2 antibody [EPR9473] (ab137069)

Overlay histogram showing HEK293 cells stained with ab137069 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137069, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - Anti-Arg2 antibody [EPR9473] (ab137069)

All lanes : Anti-Arg2 antibody [EPR9473] (ab137069) at 1/1000 dilution

Lane 1 : Caco 2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 39 kDa
Anti-Arg2 antibody [EPR9473] (ab137069)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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