Anti-Arg2 antibody [EPR9473] (ab137069) at 1/20000 dilution (purified) + Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 39 kDa



Blocking and diluting buffer : 5% NFDM/TBST

Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Arg2 with purified ab137069 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Arg2 with Purified ab137069 at 1:500 dilution (1.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Anti-Arg2 antibody [EPR9473] (ab137069) at 1/10000 dilution (purified) + JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 39 kDa



Blocking and diluting buffer : 5% NFDM/TBST

Immunofluorescent analysis of Caco 2 cells labelling Arg2 with ab137069 at 1/250 dilution.

Overlay histogram showing HEK293 cells stained with ab137069 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137069, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Arg2 antibody [EPR9473] (ab137069) at 1/1000 dilution

Lane 1 : Caco 2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 39 kDa