Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Arg2 antibody [EPR22626-51] (ab228963)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22626-51] to Arg2
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Arg2 antibody [EPR22626-51]
    See all Arg2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22626-51] to Arg2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    IP
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat small intestine and kidney lysate. IHC-P: Mouse small intestine tissue; rat kidney tissue. IP: Rat kidney lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-51] (ab228963)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-51] (ab228963)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling Arg2 with ab228963 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the mouse small intestine (PMID: 19000307) is observed. The section was incubated with ab228963 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunoprecipitation - Anti-Arg2 antibody [EPR22626-51] (ab228963)
    Immunoprecipitation - Anti-Arg2 antibody [EPR22626-51] (ab228963)

    Arg2 was immunoprecipitated from 0.35 mg rat kidney lysate with ab228963 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab228963 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

    Lane 1: Rat kidney lysate 10 µg (Input).
    Lane 2: ab228963 IP in rat kidney lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab228963 in rat kidney lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 5 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-51] (ab228963)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arg2 antibody [EPR22626-51] (ab228963)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Arg2 with ab228963 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the tubules in the rat kidney (PMID: 8954792) is observed. The section was incubated with ab228963 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Western blot - Anti-Arg2 antibody [EPR22626-51] (ab228963)
    Western blot - Anti-Arg2 antibody [EPR22626-51] (ab228963)
    All lanes : Anti-Arg2 antibody [EPR22626-51] (ab228963) at 1/1000 dilution

    Lane 1 : Mouse small intestine lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Negative control: Mouse liver (PMID: 21975054).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 seconds; Lanes 2-3: 3 minutes.

  • Western blot - Anti-Arg2 antibody [EPR22626-51] (ab228963)
    Western blot - Anti-Arg2 antibody [EPR22626-51] (ab228963)
    All lanes : Anti-Arg2 antibody [EPR22626-51] (ab228963) at 1/1000 dilution

    Lane 1 : Rat small intestine lysate
    Lane 2 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Arg2 antibody [EPR22626-51] (ab228963)
    Anti-Arg2 antibody [EPR22626-51] (ab228963)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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