Anti-PADI4 / PAD4 antibody (ab26071)
Key features and details
- Goat polyclonal to PADI4 / PAD4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PADI4 / PAD4 antibody
See all PADI4 / PAD4 primary antibodies -
Description
Goat polyclonal to PADI4 / PAD4 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
AQGTLIRVTPEQP-C
, corresponding to N terminal amino acids 2-14 of Human PADI4/ PAD4 -
Positive control
- Human spleen and human liver lysates for Western blot. Tansfected HEK293 transiently expressing PADI4 / PAD4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (ab26071)Immunohistochemical analysis of paraffin embedded Human spleen tissue staining PADI4/PAD4 using ab26071 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP staining.
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Western blot - Anti-PADI4 / PAD4 antibody (ab26071)Anti-PADI4 / PAD4 antibody (ab26071) at 0.3 µg/ml + human spleen lysate (35µg protein in RIPA buffer).
Developed using the ECL technique.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Primary incubation was 1 hour. -
Western blot - Anti-PADI4 / PAD4 antibody (ab26071)Anti-PADI4 / PAD4 antibody (ab26071) at 1 µg/ml + transfected HEK293 transiently expressing PADI4 / PAD4
Predicted band size: 74 kDa
Observed band size: 74 kDa
