Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20706] to PADI4 / PAD4
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PADI4 / PAD4 antibody [EPR20706]
See all PADI4 / PAD4 primary antibodies -
Description
Rabbit monoclonal [EPR20706] to PADI4 / PAD4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB MouseRatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse spleen tissue. WB: Mouse embryo lysate; ES-D3 whole cell lysate; HEK-293T transfected with mouse PADI4 / PAD4 expression vector containing a myc-His-tag®, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20706 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on granulocytes in rat lung.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on granulocytes in mouse spleen.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on granulocytes in mouse lung.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strongly nuclear staining on peripheral blood granulocytes of mouse spleen (PMID: 20733033, PMID: 28281906, PMID: 12393868, PMID: 12833157). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution
Lane 1 : Wild-type mouse embryonic stem cells (E14) with BSA
Lanes 2-3 : mouse embryonic stem cells (E14) PADI4 Knock out with BSA
Lysates/proteins at 40 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 74 kDa
Exposure time: 18 hours
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All lanes : Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with mouse PADI4 / PAD4 expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution
Lane 1 : Mouse embryo lysate
Lane 2 : ES-D3 (mouse embryonic multipotent stem cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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