Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
![Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141703-anti-padi4-pad4-antibody-oti4h5-western-blot.jpg "Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)")
Key features and details
- Mouse monoclonal [OTI4H5] to PADI4 / PAD4
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2a
Overview
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Product name
Anti-PADI4 / PAD4 antibody [OTI4H5]
See all PADI4 / PAD4 primary antibodies -
Description
Mouse monoclonal [OTI4H5] to PADI4 / PAD4 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-663 of Human PADI4/ PAD4 produced in HEK293T cells (NP_036519).
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Positive control
- HEK293T cells transfected with PADI4 / PAD4 , Human breast, Carcinoma of Human lung, Adenocarcinoma of Human ovary, Human pancreas, Adenocarcinoma of Human endometrium, Human prostate, Carcinoma of Human prostate, Carcinoma of Human bladder and Human lymphoma tissues, COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 , Hela and Jurkat cells
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General notes
The clone number has been updated from 4H5 to OTI4H5, both clone numbers name the same antibody clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI4H5 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)All lanes : Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086) at 1/2000 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PADI4 / PAD4 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 74 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206501) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141702-anti-padi4-pad4-antibody-oti4h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human breast tissue by Immunohistochemistry. -
![Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141693-anti-padi4-pad4-antibody-oti4h5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/100, staining COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 by Immunofluorescence. -
![Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141692-anti-padi4-pad4-antibody-oti4h5-flow-cytometry.jpg)
Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in HeLa cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometric. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141701-anti-padi4-pad4-antibody-oti4h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded carcinoma of Human lung tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141700-anti-padi4-pad4-antibody-oti4h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human ovary tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141699-anti-padi4-pad4-antibody-oti4h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human pancreas tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141698-anti-padi4-pad4-antibody-oti4h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human endometrium tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141697-anti-padi4-pad4-antibody-oti4h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human prostate tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141696-anti-padi4-pad4-antibody-oti4h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human prostate tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141695-anti-padi4-pad4-antibody-oti4h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human bladder tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141694-anti-padi4-pad4-antibody-oti4h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human lymphoma tissue by Immunohistochemistry. -
![Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)](https://www.abcam.com/ps/products/128/ab128086/Images/ab128086-141691-anti-padi4-pad4-antibody-oti4h5-flow-cytometry.jpg)
Flow Cytometry - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in Jurkat cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometric.
