Recombinant human PADI4 / PAD4 protein (ab196393)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 65% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human PADI4 / PAD4 protein
See all PADI4 / PAD4 proteins and peptides -
Biological activity
Specific activity of ≥80 pmole/min/µg.
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Purity
>= 65 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPEDCTSFSINASPGVVV DIAHGPPAKK KSTGSSTWPLDPGVEVTLTMKVASGSTGDQKVQISYYG PKTPPVKALLYLTGVEISLCAD ITRTGKVKPTRAVKDQRTWTWGPCGQ GAILLVNCDRDNLESSAMDCEDDEVLDSEDLQDM SLMTLSTKTPKDFF TNHTLVLHVARSEMDKVRVFQATRGKLSSKCSVVLGPKWPSHYLMV PG GKHNMDFYVEALAFPDTDFPGLITLTISLLDTSNLELPEAVVFQDSVVFR VAPWIMTP NTQPPQEVYACSIFENEDFLKSVTTLAMKAKCKLTICPEE ENMDDQWMQDEMEIGYIQAP HKTLPVVFDSPRNRGLKEFPIKRVMGPD FGYVTRGPQTGGISGLDSFGNLEVSPPVTVRG KEYPLGRILFGDSCYP SNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVP APDR KGFRLLLASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKT LREHNS FVERCIDWNRELLKRELGLAESDIIDIPQLFKLKEFSKAEAF FPNMVNMLVLGKHLGIPK PFGPVINGRCCLEEKVCSLLEPLGLQCTFI NDFFTYHIRHGEVHCGTNVRRKPFSFKWWN MVP -
Predicted molecular weight
75 kDa including tags -
Amino acids
1 to 663 -
Tags
DDDDK tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
Also contains 80 µg/ml DYKDDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human PADI4 / PAD4 protein (ab196393)
Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, and 2 mM DTT) containing ab196393 and 10 mM BAEE as a substrate for 25 min at 37°C. Amount of ammonia produced was measured and used for specific activity calculation.
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SDS-PAGE - Recombinant human PADI4 / PAD4 protein (ab196393)10% SDS PAGE with Coomassie staining: 2.4 µg ab196393.
