Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22006-23] to Phosphothreonine
Suitable for: WB, ELISA
Reacts with: Mouse, Rat, Human

Product name

Anti-Phosphothreonine antibody [EPR22006-23]
See all Phosphothreonine primary antibodies
Description

Rabbit monoclonal [EPR22006-23] to Phosphothreonine
Host species

Rabbit
Tested Applications & Species

Application Species
See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat, C6 and NIH/3T3, treated with Calyculin A, whole cell lysate. Jurkat, treated with pervanadate, whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22006-23
Isotype

IgG
Research areas

Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)

All lanes : Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

Lane 1 : Untreated Jurkat (human t cell leukemia T lymphocyte) whole cell lysate (Untreated membrane)
Lane 2 : Jurkat treated with 0.1 µM Calyculin A for 45 minutes, whole cell lysate (Untreated membrane)
Lane 3 : Jurkat treated with 0.1 µM Calyculin A for 45 minutes, whole cell lysate (Phosphatase treated membrane)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Observed MW: Multiple smeared bands.
Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)

All lanes : Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

Lane 1 : Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 treated with 0.1 uM Calyculin A for 15 minutes, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Exposure time: 59 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Observed MW: Multiple smeared bands.
Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)

All lanes : Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

Lane 1 : Untreated C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : C6 treated with 0.1 uM Calyculin A for 30 minutes, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Observed MW: Multiple smeared bands.
Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)

All lanes : Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

Lane 1 : Untreated Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 20 µg
Lane 2 : Jurkat treated with 50 mM pervanadate for 5 minutes, whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Exposure time: 6 seconds

No difference in the signal detected by ab218195 upon pervanadate (a protein tyrosine phosphatase inhibitor) treatment.

Blocking/Dilution buffer: 5% NFDM/TBST.
ELISA - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)

ELISA - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195).

Each point in the Y axis represents one peptide of the corresponding protein used in ELISA test. The red asterisk indicates a phosphorylated amino acid site. The X axis is the signal to noise (S/N) ratio calculated by the signal intensity of a phospho peptide and its non-phospho paralog.

Antigens used at 1 µg/ml: TWIST1(pY107); TP73(pY99); SQSTM1 (pS403); NFKB (pS337); Phosphoserine; Phosphothreonine; ADD1(pT445); EIF2AK3(pT982); AURKB(pT232); AKT1(pT308).

Primary antibody ab218195 used at a 1/10,000 dilution.

Secondary antibody Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) used at a 1/2,500 dilution.
Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)

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