Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22006-23] to Phosphothreonine
  • Suitable for: WB, ELISA
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Phosphothreonine antibody [EPR22006-23]
    See all Phosphothreonine primary antibodies

  • Description

    Rabbit monoclonal [EPR22006-23] to Phosphothreonine

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, C6 and NIH/3T3, treated with Calyculin A, whole cell lysate. Jurkat, treated with pervanadate, whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)
    Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)
    All lanes : Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

    Lane 1 : Untreated Jurkat (human t cell leukemia T lymphocyte) whole cell lysate (Untreated membrane)
    Lane 2 : Jurkat treated with 0.1 µM Calyculin A for 45 minutes, whole cell lysate (Untreated membrane)
    Lane 3 : Jurkat treated with 0.1 µM Calyculin A for 45 minutes, whole cell lysate (Phosphatase treated membrane)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed MW: Multiple smeared bands.

  • Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)
    Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)
    All lanes : Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 2 : NIH/3T3 treated with 0.1 uM Calyculin A for 15 minutes, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Exposure time: 59 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed MW: Multiple smeared bands.

  • Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)
    Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)
    All lanes : Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

    Lane 1 : Untreated C6 (rat glial tumor glial cell) whole cell lysate
    Lane 2 : C6 treated with 0.1 uM Calyculin A for 30 minutes, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed MW: Multiple smeared bands.

  • Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)
    Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)
    All lanes : Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

    Lane 1 : Untreated Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 20 µg
    Lane 2 : Jurkat treated with 50 mM pervanadate for 5 minutes, whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Exposure time: 6 seconds


    No difference in the signal detected by ab218195 upon pervanadate (a protein tyrosine phosphatase inhibitor) treatment.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ELISA - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)
    ELISA - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)

    ELISA - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195).

    Each point in the Y axis represents one peptide of the corresponding protein used in ELISA test. The red asterisk indicates a phosphorylated amino acid site. The X axis is the signal to noise (S/N) ratio calculated by the signal intensity of a phospho peptide and its non-phospho paralog.

    Antigens used at 1 µg/ml: TWIST1(pY107); TP73(pY99); SQSTM1 (pS403); NFKB (pS337); Phosphoserine; Phosphothreonine; ADD1(pT445); EIF2AK3(pT982); AURKB(pT232); AKT1(pT308).

    Primary antibody ab218195 used at a 1/10,000 dilution.

    Secondary antibody Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) used at a 1/2,500 dilution.

  • Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)
    Anti-Phosphothreonine antibody [EPR22006-23] (ab218195)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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