Human Laminin alpha 4/LAMA4 ELISA Kit (ab277457)
Key features and details
- Sensitivity: 1.63 ng/ml
- Range: 1.638 ng/ml - 400 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Laminin alpha 4/LAMA4 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.63 ng/ml -
Range
1.638 ng/ml - 400 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 122.1 89% - 142% Plasma 119.2 97% - 149% Cell culture media 117.8 97% - 140% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Laminin alpha 4/LAMA4 ELISA Kit (ab277457) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Laminin alpha 4/LAMA4 in serum (human Laminin alpha 4 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human Laminin alpha 4/LAMA4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Laminin alpha 4/LAMA4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Laminin alpha 4/LAMA4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Laminin alpha 4/LAMA4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated Anti-Human Laminin alpha 4/LAMA4 Antibody 2 vials Anti-Human Laminin alpha 4 LAMA4 coated Microplate 1 unit Human Laminin alpha 4/LAMA4 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
In adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn tissues is observed in certain mesenchymal cells in tissues such as smooth muscle and dermis. -
Involvement in disease
Cardiomyopathy, dilated 1JJ -
Sequence similarities
Contains 4 laminin EGF-like domains.
Contains 5 laminin G-like domains. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular. -
Cellular localization
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
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Alternative names
- CMD1JJ
- DKFZp686D23145
- LAMA3
see all -
Database links
- Entrez Gene: 3910 Human
- Omim: 600133 Human
- SwissProt: Q16363 Human
- Unigene: 654572 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.