Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5578] to Integrin alpha 6 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free
    See all Integrin alpha 6 primary antibodies

  • Description

    Rabbit monoclonal [EPR5578] to Integrin alpha 6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SW480, HEK293, HT-1376, JAR and human platelet lysates.

  • General notes

    ab248060 is the carrier-free version of ab124924 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248060 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)
    Western blot - Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)
    All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution

    Lane 1 : Wild-type SW480 cell lysate
    Lane 2 : ITGA6 knockout SW480 cell lysate
    Lane 3 : HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 126 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab124924).

    Lanes 1 - 3: Merged signal (red and green). Green - ab124924 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab124924 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout sample. Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124924 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)
    Western blot - Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)
    All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/10000 dilution (purified)

    Lane 1 : HT-1376 cell lysate
    Lane 2 : JAR cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 126 kDa
    Observed band size: 126 kDa



    This data was developed using ab124924, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)
    Western blot - Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)
    All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : HT1376 cell lysate
    Lane 3 : Human platelet lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 126 kDa



    This data was developed using ab124924, the same antibody clone in a different buffer formulation.

    Secondary antibody - anti-rabbit HRP (ab6721).

  • OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)
    OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)
    This data was developed using ab124924, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)
    Anti-Integrin alpha 6 antibody [EPR5578] - BSA and Azide free (ab248060)

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