Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µg 304 886 ₸

Order now and get it on Wednesday March 24, 2021

Anti-Phosphothreonine antibody [RM102] (ab238305)

Key features and details

Rabbit monoclonal [RM102] to Phosphothreonine
Suitable for: ELISA, WB, IP, ICC/IF
Reacts with: Species independent
Isotype: IgG

Product name

Anti-Phosphothreonine antibody [RM102]
See all Phosphothreonine primary antibodies
Description

Rabbit monoclonal [RM102] to Phosphothreonine
Host species

Rabbit
Specificity

ab238305 reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
Tested applications

Suitable for: ELISA, WB, IP, ICC/IFmore details
Species reactivity

Reacts with: Species independent
Immunogen

Synthetic peptide corresponding to Phosphothreonine conjugated to bovine serum albumin. Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide.
Positive control
- ICC/IF: Serum-starved A431 cells treated with Calyculin A/Okadaic Acid. WB: Serum-starved A431 cells treated with Calyculin A/Okadaic Acid. IP: A431 cells treated with Calyculin A/Okadaic Acid.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from an animal origin–free culture supernatant.
Clonality

Monoclonal
Clone number

RM102
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Phosphothreonine antibody [RM102] (ab238305)

Serum-starved A431 (human epidermoid carcinoma cell line) cells treated with Calyculin A/Okadaic Acid stained for Phosphothreonine using ab238305 at a 1/500 dilution in ICC/IF, followed by a PE conjugated secondary antibody (red). Counterstained with DAPI (blue).

Left panel: Non-treated cells.

Right panel: Cells treated with Calyculin A/Okadaic Acid.
Western blot - Anti-Phosphothreonine antibody [RM102] (ab238305)

All lanes : Anti-Phosphothreonine antibody [RM102] (ab238305) at 1/2000 dilution

Lane 1 : Serum starved A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : Serum starved A431 cells treated with Calyculin A/Okadaic Acid lysate
Immunoprecipitation - Anti-Phosphothreonine antibody [RM102] (ab238305)

Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 (Human epidermoid carcinoma cell line) cells by ab238305 at a 1/500 dilution, and then blotted with ab238305.

Lane 1: Whole lysate control.
Lane 2: IP with rabbit IgG control.
Lane 3: IP with ab238305.
ELISA - Anti-Phosphothreonine antibody [RM102] (ab238305)

ab238305 recognizes Phosphorylated threonine in peptides with different sequences.

It has minimal cross-reactivity with phosphorylated serine.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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