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Anti-Phosphothreonine antibody [RM102] (ab238305)

Key features and details

  • Rabbit monoclonal [RM102] to Phosphothreonine
  • Suitable for: ELISA, WB, IP, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG

Overview

  • Product name

    Anti-Phosphothreonine antibody [RM102]
    See all Phosphothreonine primary antibodies

  • Description

    Rabbit monoclonal [RM102] to Phosphothreonine

  • Host species

    Rabbit

  • Specificity

    ab238305 reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.

  • Tested applications

    Suitable for: ELISA, WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide corresponding to Phosphothreonine conjugated to bovine serum albumin. Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide.

  • Positive control

    • ICC/IF: Serum-starved A431 cells treated with Calyculin A/Okadaic Acid. WB: Serum-starved A431 cells treated with Calyculin A/Okadaic Acid. IP: A431 cells treated with Calyculin A/Okadaic Acid.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Phosphothreonine antibody [RM102] (ab238305)
    Immunocytochemistry/ Immunofluorescence - Anti-Phosphothreonine antibody [RM102] (ab238305)

    Serum-starved A431 (human epidermoid carcinoma cell line) cells treated with Calyculin A/Okadaic Acid stained for Phosphothreonine using ab238305 at a 1/500 dilution in ICC/IF, followed by a PE conjugated secondary antibody (red). Counterstained with DAPI (blue).

    Left panel: Non-treated cells.

    Right panel: Cells treated with Calyculin A/Okadaic Acid.

  • Western blot - Anti-Phosphothreonine antibody [RM102] (ab238305)
    Western blot - Anti-Phosphothreonine antibody [RM102] (ab238305)
    All lanes : Anti-Phosphothreonine antibody [RM102] (ab238305) at 1/2000 dilution

    Lane 1 : Serum starved A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 2 : Serum starved A431 cells treated with Calyculin A/Okadaic Acid lysate
  • Immunoprecipitation - Anti-Phosphothreonine antibody [RM102] (ab238305)
    Immunoprecipitation - Anti-Phosphothreonine antibody [RM102] (ab238305)

    Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 (Human epidermoid carcinoma cell line) cells by ab238305 at a 1/500 dilution, and then blotted with ab238305.

    Lane 1: Whole lysate control.
    Lane 2: IP with rabbit IgG control.
    Lane 3: IP with ab238305.

  • ELISA - Anti-Phosphothreonine antibody [RM102] (ab238305)
    ELISA - Anti-Phosphothreonine antibody [RM102] (ab238305)

    ab238305 recognizes Phosphorylated threonine in peptides with different sequences.

    It has minimal cross-reactivity with phosphorylated serine.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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