Anti-PFAS antibody - N-terminal (ab186048)
Key features and details
- Rabbit polyclonal to PFAS - N-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PFAS antibody - N-terminal
See all PFAS primary antibodies -
Description
Rabbit polyclonal to PFAS - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human PFAS aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_036525.1).
Database link: O15067 -
Positive control
- Jurkat, HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab186048 was affinity purified using an epitope specific to PFAS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PFAS antibody - N-terminal (ab186048)All lanes : Anti-PFAS antibody - N-terminal (ab186048) at 1 µg/ml
Lane 1 : Jurkat whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : 293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 145 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-PFAS antibody - N-terminal (ab186048)Detection of PFAS in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186048 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents rabbit IgG IP control.
Detection: Chemiluminescence with an exposure time of 3 minutes.
