Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)
![Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)](https://www.abcam.com/ps/products/256/ab256533/Images/ab256533-352719-ELISA1anti-phosphothreonine-antibody-epr22006-23-elisa.jpg "Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22006-23] to Phosphothreonine - BSA and Azide free
- Suitable for: WB, ELISA
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free
See all Phosphothreonine primary antibodies -
Description
Rabbit monoclonal [EPR22006-23] to Phosphothreonine - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, C6 and NIH/3T3, treated with Calyculin A, whole cell lysate. Jurkat, treated with pervanadate, whole cell lysate.
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General notes
ab256533 is the carrier-free version of ab218195 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab256533 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22006-23 -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)
ELISA - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195).
Each point in the Y axis represents one peptide of the corresponding protein used in ELISA test. The red asterisk indicates a phosphorylated amino acid site. The X axis is the signal to noise (S/N) ratio calculated by the signal intensity of a phospho peptide and its non-phospho paralog.
Antigens used at 1 µg/ml: TWIST1(pY107); TP73(pY99); SQSTM1 (pS403); NFKB (pS337); Phosphoserine; Phosphothreonine; ADD1(pT445); EIF2AK3(pT982); AURKB(pT232); AKT1(pT308).
Primary antibody ab218195 used at a 1/10,000 dilution.
Secondary antibody Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) used at a 1/2,500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218195).
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![Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)](https://www.abcam.com/ps/products/256/ab256533/Images/ab256533-2-benefits-of-recombinant-antibodies.png)
Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)
