Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22006-23] to Phosphothreonine - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free
    See all Phosphothreonine primary antibodies

  • Description

    Rabbit monoclonal [EPR22006-23] to Phosphothreonine - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISAmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, C6 and NIH/3T3, treated with Calyculin A, whole cell lysate. Jurkat, treated with pervanadate, whole cell lysate.

  • General notes

    ab256533 is the carrier-free version of ab218195 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab256533 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ELISA - Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)
    ELISA - Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)

    ELISA - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195).

    Each point in the Y axis represents one peptide of the corresponding protein used in ELISA test. The red asterisk indicates a phosphorylated amino acid site. The X axis is the signal to noise (S/N) ratio calculated by the signal intensity of a phospho peptide and its non-phospho paralog.

    Antigens used at 1 µg/ml: TWIST1(pY107); TP73(pY99); SQSTM1 (pS403); NFKB (pS337); Phosphoserine; Phosphothreonine; ADD1(pT445); EIF2AK3(pT982); AURKB(pT232); AKT1(pT308).

    Primary antibody ab218195 used at a 1/10,000 dilution.

    Secondary antibody Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) used at a 1/2,500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218195).

  • Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)
    Anti-Phosphothreonine antibody [EPR22006-23] - BSA and Azide free (ab256533)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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