Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10807(B)] to CHMP2B - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt (Intra), IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free
    See all CHMP2B primary antibodies

  • Description

    Rabbit monoclonal [EPR10807(B)] to CHMP2B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt (Intra), IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type A549 whole cell lysate, Hela, 293T, MCF7 whole cell lysates; Mouse brain lysate, Rat brain lysate, Rat heart lysate ICC/IF: HepG2 cells; Flow cyt: 293T cells; IP: 293T whole cell lysate.

  • General notes

    ab249311 is the carrier-free version of ab157208.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    Western blot - Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    All lanes : Anti-CHMP2B antibody [EPR10807(B)] (ab157208) at 1/1000 dilution

    Lane 1 : Wild-type A549 whole cell lysate
    Lane 2 : CHMP2B knockout A549 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 24 kDa



    This data was developed using ab157208, the same antibody clone in a different buffer formulation.

    Lanes 1 - 4: Merged signal (red and green). Green - ab157208 observed at 45 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab157208 was shown to specifically react with CHMP2B in wild-type A549 cells as signal was lost in CHMP2B knockout cells. Wild-type and CHMP2B knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab157208 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    Immunocytochemistry - Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    This data was developed using ab157208, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HepG2 cells, labeling CHMP2B using ab157208 at a 1/250 dilution.
  • Western blot - Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    Western blot - Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    All lanes : Anti-CHMP2B antibody [EPR10807(B)] (ab157208) at 1/1000 dilution

    Lane 1 : Fetal brain tissue lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 24 kDa



    This data was developed using ab157208, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    Immunoprecipitation - Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    This data was developed using ab157208, the same antibody clone in a different buffer formulation.Western blot analysis of Immunoprecipitation pellet from Human fetal brain lysate showing CHMP2B, using ab157208 at 1/10 dilution for IP
  • Flow Cytometry - Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    Flow Cytometry - Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    This data was developed using ab157208, the same antibody clone in a different buffer formulation.Flow cytometry analysis of permeabilized 293T cells labeling CHMP2B (red), using ab157208 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
  • Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)
    Anti-CHMP2B antibody [EPR10807(B)] - BSA and Azide free (ab249311)

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