Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12334] to RBP2 - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-RBP2 antibody [EPR12334] - BSA and Azide free
See all RBP2 primary antibodies -
Description
Rabbit monoclonal [EPR12334] to RBP2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250197 is the carrier-free version of ab180494.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12334 -
Isotype
IgG -
Research areas
Images
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Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution + Human small intestine tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 16 kDaThis data was developed using ab180494, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution + Caco-2 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Predicted band size: 16 kDaThis data was developed using ab180494, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab180494, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling RBP2 with ab180494 at a 1/500 dilution. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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This data was developed using ab180494, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cell line staining RBP2 using ab180494 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/250. Counter stain: Dapi.
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