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Signal Transduction Metabolism Vitamins / Minerals

Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)

Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12334] to RBP2 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-RBP2 antibody [EPR12334] - BSA and Azide free
    See all RBP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12334] to RBP2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250197 is the carrier-free version of ab180494.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12334
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals

Images

  • Western blot - Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
    Western blot - Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
    Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution + Human small intestine tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 16 kDa



    This data was developed using ab180494, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
    Western blot - Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
    Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution + Caco-2 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

    Predicted band size: 16 kDa



    This data was developed using ab180494, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
    This data was developed using ab180494, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling RBP2 with ab180494 at a 1/500 dilution. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
    Immunocytochemistry - Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
    This data was developed using ab180494, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cell line staining RBP2 using ab180494 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/250. Counter stain: Dapi.
  • Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)
    Anti-RBP2 antibody [EPR12334] - BSA and Azide free (ab250197)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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