Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ATP7b antibody [EPR6794] (ab124973)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6794] to ATP7b
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-ATP7b antibody [EPR6794]
See all ATP7b primary antibodies
Description

Rabbit monoclonal [EPR6794] to ATP7b
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human ATP7b aa 1400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P35670
Positive control
- WB: Caco-2 and HepG2 whole cell lysate (ab7900). Flow Cyt: HepG2 cells. ICC/IF: HepG2 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.40 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6794
Isotype

IgG
Research areas

Western blot - Anti-ATP7b antibody [EPR6794] (ab124973)

Anti-ATP7b antibody [EPR6794] (ab124973) at 1/10000 dilution (Purified) + Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 157 kDa
Observed band size: 157 kDa
Western blot - Anti-ATP7b antibody [EPR6794] (ab124973)

Anti-ATP7b antibody [EPR6794] (ab124973) at 1/1000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 157 kDa
Observed band size: 157 kDa
Flow Cytometry - Anti-ATP7b antibody [EPR6794] (ab124973)

Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATP7b with purified ab124973 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody [EPR6794] (ab124973)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATP7b with purified ab124973 at 1:100 dilution (1.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-ATP7b antibody [EPR6794] (ab124973)

Flow cytometric analysis of permeabilized HepG2 cells using unpurified anti-ATP7B RabMAb in red (ab124973) or a rabbit IgG in green(negative).
OI-RD Scanning - Anti-ATP7b antibody [EPR6794] (ab124973)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-ATP7b antibody [EPR6794] (ab124973)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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