Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP7b antibody [EPR6794] (ab124973)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6794] to ATP7b
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-ATP7b antibody [EPR6794]
    See all ATP7b primary antibodies

  • Description

    Rabbit monoclonal [EPR6794] to ATP7b

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ATP7b aa 1400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P35670

  • Positive control

    • WB: Caco-2 and HepG2 whole cell lysate (ab7900). Flow Cyt: HepG2 cells. ICC/IF: HepG2 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ATP7b antibody [EPR6794] (ab124973)
    Western blot - Anti-ATP7b antibody [EPR6794] (ab124973)
    Anti-ATP7b antibody [EPR6794] (ab124973) at 1/10000 dilution (Purified) + Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 157 kDa
    Observed band size: 157 kDa

  • Western blot - Anti-ATP7b antibody [EPR6794] (ab124973)
    Western blot - Anti-ATP7b antibody [EPR6794] (ab124973)
    Anti-ATP7b antibody [EPR6794] (ab124973) at 1/1000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 157 kDa
    Observed band size: 157 kDa

  • Flow Cytometry - Anti-ATP7b antibody [EPR6794] (ab124973)
    Flow Cytometry - Anti-ATP7b antibody [EPR6794] (ab124973)

    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATP7b with purified ab124973 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody [EPR6794] (ab124973)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody [EPR6794] (ab124973)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATP7b with purified ab124973 at 1:100 dilution (1.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-ATP7b antibody [EPR6794] (ab124973)
    Flow Cytometry - Anti-ATP7b antibody [EPR6794] (ab124973)

    Flow cytometric analysis of permeabilized HepG2 cells using unpurified anti-ATP7B RabMAb in red (ab124973) or a rabbit IgG in green(negative).

  • OI-RD Scanning - Anti-ATP7b antibody [EPR6794] (ab124973)
    OI-RD Scanning - Anti-ATP7b antibody [EPR6794] (ab124973)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ATP7b antibody [EPR6794] (ab124973)
    Anti-ATP7b antibody [EPR6794] (ab124973)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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