Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RBP2 antibody [EPR12334] (ab180494)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12334] to RBP2
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-RBP2 antibody [EPR12334]
    See all RBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12334] to RBP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human small intestine and Caco2; Human small intestine tissue; SW480 cell line.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RBP2 antibody [EPR12334] (ab180494)
    Western blot - Anti-RBP2 antibody [EPR12334] (ab180494)
    Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution + Human small intestine at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 16 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-RBP2 antibody [EPR12334] (ab180494)
    Western blot - Anti-RBP2 antibody [EPR12334] (ab180494)
    Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution + Caco2 at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

    Predicted band size: 16 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] (ab180494)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] (ab180494)

    Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling RBP2 with ab180494 at a 1/500 dilution. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RBP2 antibody [EPR12334] (ab180494)
    Immunocytochemistry/ Immunofluorescence - Anti-RBP2 antibody [EPR12334] (ab180494)

    Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cell line staining RBP2 using ab180494 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/250. Counter stain: Dapi.

  • Anti-RBP2 antibody [EPR12334] (ab180494)
    Anti-RBP2 antibody [EPR12334] (ab180494)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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