Anti-RBP2 antibody [EPR12334] (ab180494)
![Anti-RBP2 antibody [EPR12334] (ab180494)](https://www.abcam.com/ps/products/180/ab180494/Images/ab180494-211959-anti-rbp2-antibody-epr12334-western-blot.jpg "Anti-RBP2 antibody [EPR12334] (ab180494)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12334] to RBP2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-RBP2 antibody [EPR12334]
See all RBP2 primary antibodies -
Description
Rabbit monoclonal [EPR12334] to RBP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human small intestine and Caco2; Human small intestine tissue; SW480 cell line.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12334 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RBP2 antibody [EPR12334] (ab180494)Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution + Human small intestine at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 16 kDaBlocking buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-RBP2 antibody [EPR12334] (ab180494)](https://www.abcam.com/ps/products/180/ab180494/Images/ab180494-211960-anti-rbp2-antibody-epr12334-western-blot.jpg)
Western blot - Anti-RBP2 antibody [EPR12334] (ab180494)Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution + Caco2 at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Predicted band size: 16 kDaBlocking buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] (ab180494)](https://www.abcam.com/ps/products/180/ab180494/Images/ab180494-211961-anti-rbp2-antibody-epr12334-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] (ab180494)Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling RBP2 with ab180494 at a 1/500 dilution. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-RBP2 antibody [EPR12334] (ab180494)](https://www.abcam.com/ps/products/180/ab180494/Images/ab180494-211962-anti-rbp2-antibody-epr12334-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RBP2 antibody [EPR12334] (ab180494)Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cell line staining RBP2 using ab180494 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/250. Counter stain: Dapi.
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![Anti-RBP2 antibody [EPR12334] (ab180494)](https://www.abcam.com/ps/products/180/ab180494/Images/ab180494-5-benefits-of-recombinant-antibodies.png)
Anti-RBP2 antibody [EPR12334] (ab180494)
