Antibodies, Proteins, Kits and Reagents for Life Science

247 259 ₸ Product size

100 µl 247 259 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15629] to TEAD4/TEF-1
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-TEAD4/TEF-1 antibody [EPR15629]
See all TEAD4/TEF-1 primary antibodies
Description

Rabbit monoclonal [EPR15629] to TEAD4/TEF-1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HEK293T, HeLa and SW480 whole cell lysates; Human TEAD1, TEAD2, TEAD3 and TEAD4/TEF-1 recombinant proteins (GST-Tag).
General notes
This product was previously labelled as TEAD4

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15629
Isotype

IgG

Western blot - Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)

All lanes : Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : TEAD4 knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 48 kDa
Observed band size: 54 kDa
why is the actual band size different from the predicted?

Lanes 1- 2: Merged signal (red and green). Green - ab197589 observed at 54 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab197589 was shown to react with TEAD4/TEF-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266185 (knockout cell lysate ab257732) was used. Wild-type HEK-293T and TEAD4 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab197589 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)

All lanes : Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)

All lanes : Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589) at 1/5000 dilution

Lane 1 : Human TEAD4/TEF-1 recombinant protein (GST-Tag) aa165-280
Lane 2 : Human TEAD4/TEF-1 recombinant protein (GST-Tag) aa161-280
Lane 3 : Human TEAD2 recombinant protein (GST-Tag) aa161-290
Lane 4 : Human TEAD3 recombinant protein (GST-Tag) aa161-290

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 48 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)

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