Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15629] to TEAD4/TEF-1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TEAD4/TEF-1 antibody [EPR15629]
    See all TEAD4/TEF-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15629] to TEAD4/TEF-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HEK293T, HeLa and SW480 whole cell lysates; Human TEAD1, TEAD2, TEAD3 and TEAD4/TEF-1 recombinant proteins (GST-Tag).

  • General notes

     

     

     This product was previously labelled as TEAD4

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15629

  • Isotype

    IgG

Images

  • Western blot - Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)
    Western blot - Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)
    All lanes : Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : TEAD4 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab197589 observed at 54 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab197589 was shown to react with TEAD4/TEF-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266185 (knockout cell lysate ab257732) was used. Wild-type HEK-293T and TEAD4 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab197589 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)
    Western blot - Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)
    All lanes : Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)
    Western blot - Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)
    All lanes : Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589) at 1/5000 dilution

    Lane 1 : Human TEAD4/TEF-1 recombinant protein (GST-Tag) aa165-280
    Lane 2 : Human TEAD4/TEF-1 recombinant protein (GST-Tag) aa161-280
    Lane 3 : Human TEAD2 recombinant protein (GST-Tag) aa161-290
    Lane 4 : Human TEAD3 recombinant protein (GST-Tag) aa161-290

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 48 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)
    Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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