Human Pentraxin 3 / PTX3 ELISA Kit (ab214570)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.4 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Pentraxin 3 / PTX3 ELISA Kit
See all Pentraxin 3/PTX3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma-EDTA 5 6.1% Inter-assay Sample n Mean SD CV% Plasma-EDTA 3 3.7% -
Sample type
Cell culture supernatant, Saliva, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.4 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 100 93% - 105% Saliva 92 88% - 95% Serum 104 100% - 108% Cell culture extracts 100 93% - 103% Hep Plasma 99 98% - 100% EDTA Plasma 82 80% - 85% Cit plasma 101 94% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Pentraxin 3 / PTX3 ELISA Kit (ab214570) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pentraxin 3 / PTX3 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, tissue extracts, and saliva. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Pentraxin 3 / PTX3 with 3.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Pentraxin 3 (also known as pentraxin-related protein PTX3 or TSG-14) is a secreted protein with a role in innate immune response. It is secreted by many cell types during infectious situations. The standard protein provided in this kit corresponds to the C-terminal Pentaxin domain of Pentraxin 3.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Pentraxin 3 Capture Antibody 1 x 600µl 10X Human Pentraxin 3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Pentraxin 3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. -
Sequence similarities
Contains 1 pentaxin domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- AI607804
- Pentaxin 3
- Pentaxin-related protein PTX3
see all -
Database links
- Entrez Gene: 5806 Human
- Omim: 602492 Human
- SwissProt: P26022 Human
- Unigene: 591286 Human
Images
-
Other - Human Pentraxin 3 / PTX3 ELISA Kit (ab214570)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Human Pentraxin 3 standard curve comparison data.Standard curve comparison between human Pentraxin 3 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 33-fold increase in sensitivity.
-
Example of human Pentraxin 3 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Example of human Pentraxin 3 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Pentraxin 3 in human plasma, saliva and cell culture supernatant samples.The concentrations of Pentraxin 3 were measured in duplicates, interpolated from the Pentraxin 3 standard curves and corrected for sample dilution. Undiluted samples are as follows: Plasma (heparin) 25%, plasma (EDTA) 25%, saliva 10%, and HUVEC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pentraxin 3 concentration was determined to be 1,056 pg/mL in plasma (heparin), 1,329 og/mL in plasma (EDTA), 962 pg/mL in saliva and 334 pg/mL in HUVEC cell culture supernatant.
-
Interpolated concentrations of spiked Pentraxin 3 in human serum and plasma samples.The concentrations of Pentraxin 3 were measured in duplicates, interpolated from the Pentraxin 3 standard curves and corrected for sample dilution. Undiluted samples are as follows: Serum 50%, and Plasma (Citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Interpolated concentrations of native Pentraxin 3 in human HUVEC Extract sample based on a 200 µg/mL extract load.The concentrations of Pentraxin 3 were measured in duplicate and interpolated from the Pentraxin 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pentraxin 3 concentration was determined to be 1,368 pg/mL.
-
Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (ab214570)To learn more about the advantages of recombinant antibodies see here.
