Anti-CENPE antibody [EPR4543(2)] (ab124733)
![Anti-CENPE antibody [EPR4543(2)] (ab124733)](https://www.abcam.com/ps/products/124/ab124733/Images/ab124733-256169-anti-cenpe-antibody-epr45432-western-blot.jpg "Anti-CENPE antibody [EPR4543(2)] (ab124733)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4543(2)] to CENPE
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-CENPE antibody [EPR4543(2)]
See all CENPE primary antibodies -
Description
Rabbit monoclonal [EPR4543(2)] to CENPE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide within Human CENPE aa 2500-2600. The exact sequence is proprietary.
-
Positive control
- HepG2, HeLa, and Jurkat cell lysates.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4543(2) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-CENPE antibody [EPR4543(2)] (ab124733)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CENPE knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124733 observed at 310 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124733 was shown to specifically react with CENPE when CENPE knockout samples were used. Wild-type and CENPE knockout samples were subjected to SDS-PAGE. ab124733 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-CENPE antibody [EPR4543(2)] (ab124733)](https://www.abcam.com/ps/products/124/ab124733/Images/ab124733-138563-anti-cenpe-antibody-epr45432-western-blot.jpg)
Western blot - Anti-CENPE antibody [EPR4543(2)] (ab124733)All lanes : Anti-CENPE antibody [EPR4543(2)] (ab124733) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 316 kDa
-
![Anti-CENPE antibody [EPR4543(2)] (ab124733)](https://www.abcam.com/ps/products/124/ab124733/Images/ab124733-3-benefits-of-recombinant-antibodies.png)
Anti-CENPE antibody [EPR4543(2)] (ab124733)
