Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CENPE antibody [EPR4542(2)] (ab133583)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4542(2)] to CENPE
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CENPE antibody [EPR4542(2)]
    See all CENPE primary antibodies

  • Description

    Rabbit monoclonal [EPR4542(2)] to CENPE

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to an internal sequence within amino acids 10-50 of Human CENPE (UniProt Q02224).

  • Positive control

    • HeLa treated with aphidicolin and Nocodazole, HepG2 and Jurkat cell lysates

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583)
    Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CENPE knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133583 observed at 310 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab133583 was shown to specifically react with CENPE when CENPE knockout samples were used. Wild-type and CENPE knockout samples were subjected to SDS-PAGE. ab133583 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583)
    Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583)
    All lanes : Anti-CENPE antibody [EPR4542(2)] (ab133583) at 1/1000 dilution

    Lane 1 : HeLa treated with aphidicolin and Nocodazole cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 312 kDa

  • OI-RD Scanning - Anti-CENPE antibody [EPR4542(2)] (ab133583)
    OI-RD Scanning - Anti-CENPE antibody [EPR4542(2)] (ab133583)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CENPE antibody [EPR4542(2)] (ab133583)
    Anti-CENPE antibody [EPR4542(2)] (ab133583)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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