Magnesium Assay Kit (ab102506)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 40 µM
Overview
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Product name
Magnesium Assay Kit
See all Magnesium kits -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Quantitative -
Sensitivity
40 µM -
Range
2 µM - 15 µM -
Assay time
0h 30m -
Product overview
Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to Mg2+ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity ~40 µM.
Visit our FAQs page for tips and troubleshooting. -
Notes
Magnesium is the 11th most abundant element by mass in the human body. Mg2+ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg2+ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg2+ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Magnesium Assay Buffer WM 1 x 25ml Magnesium Developer Purple 1 unit Magnesium Enzyme Mix Green 1 unit Magnesium Standard Yellow 1 x 0.1ml -
Research areas
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Relevance
Magnesium is the 11th most abundant element by mass in the human body. Magnesium is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Magnesium is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Magnesium compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia. -
Alternative names
- Mg++
- Mg+2
- Mg2+
Images
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Functional Studies - Magnesium Detection Kit (ab102506)
Magnesium measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
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Functional Studies - Magnesium Detection Kit (ab102506)Magnesium measured in biological fluids showing quantity (nmol) per microliter of tested sample
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Functional Studies - Magnesium Detection Kit (ab102506)Colourimetric standard curve : mean of duplicates (+/-SD) with background reads substracted
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Functional Studies - Magnesium Detection Kit (ab102506)Magnesium standard curve: Assay is performed according to kit protocol. Vertical dotted lines indicate the lower and upper limits of normal serum Magnesium concentrations.