Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1665Y] to Endo G - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Endo G antibody [EP1665Y] - BSA and Azide free
    See all Endo G primary antibodies

  • Description

    Rabbit monoclonal [EP1665Y] to Endo G - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247443 is the carrier-free version of ab76122. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247443 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)
    Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)

    This data was developed using ab76122, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Endo G HAP1 cell lysate (20 µg)

    Lane 3: Mouse liver tissue lysate (20 µg)

    Lane 4: HepG2 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.

    ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)
    Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)
    Anti-Endo G antibody [EP1665Y] (ab76122) at 1/2000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76122, the same antibody clone in a different buffer formulation.

     

  • Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)
    Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)

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