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Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)

Key features and details

  • Mouse monoclonal [12A668] to RANKL - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-RANKL antibody [12A668] - BSA and Azide free
    See all RANKL primary antibodies

  • Description

    Mouse monoclonal [12A668] to RANKL - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Mouse RANKL aa 1-317.
    Database link: O14788

  • Positive control

    • IHC-P: Human lymph node and liver tissue. ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) cells. WB: Human lymph node lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
    Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
    Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039) at 2 µg + transfected cell lysate

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)

    Paraffin embedded human lymph node tissue stained for RANKL with ab45039 at 5 µg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
    Immunocytochemistry/ Immunofluorescence - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)

    ICC/IF image of ab45039 stained HeLa cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45039, 10 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)

    Paraffin embedded human liver tissue stained for RANKL with ab45039 at a 1/500 dilution. Peroxidase-conjugate and DAB chromogen. A 2 hour incubation at RT was used.

  • Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
    Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
    Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039) at 0.5 µg/ml + human lymph node lysate in RIPA buffer at 35 µg

    Predicted band size: 35 kDa
    Additional bands at: 28 kDa (possible isoform)



    Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_003692.1 and of 27.7kDa according to NP_143026.1)

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