Anti-RANKL antibody (ab9957)
Key features and details
- Rabbit polyclonal to RANKL
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: unknown
Overview
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Product name
Anti-RANKL antibody
See all RANKL primary antibodies -
Description
Rabbit polyclonal to RANKL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragmentWB Human
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Immunogen
Highly pure (>98%) recombinant hsRANK-L (human soluble receptor activator of NF-Kappa B Ligand)
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General notes
Although some customers have been successful in IHC we no longer batch test in this application.
We have received both positive and negative customer feedback on mouse reactivity for this antibody. Therefore, we do not guarantee this species.
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-RANKL antibody (ab9957)Anti-RANKL antibody (ab9957) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
RANKL contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Sandwich ELISA - Anti-RANKL antibody (ab9957)To detect human sRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83145 is required. This biotinylated polyclonal antibody, in conjunction with an anti-human sRANKL (ab9957) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human sRANKL.
