Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (ab247429)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3145] to Rad17 (phospho S656) - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free
    See all Rad17 primary antibodies

  • Description

    Rabbit monoclonal [EPR3145] to Rad17 (phospho S656) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects Rad17 phosphoryated on Serine 656.

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247429 is the carrier-free version of ab76012 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247429 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (ab247429)
    Western blot - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (ab247429)
    All lanes : Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012) at 1/10000 dilution

    Lane 1 : HeLa cell lysate, untreated
    Lane 2 : HeLa cell lysate treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 77 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76012, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (ab247429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (ab247429)
    This data was developed using ab76012, the same antibody clone in a different buffer formulation.ab76012, at 1/100-1/250 dilution, staining Rad17 in human breast carcinoma by immunohistochemistry using paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (ab247429)
    Immunocytochemistry - Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (ab247429)
    This data was developed using ab76012, the same antibody clone in a different buffer formulation.ab76012, at 1/100-1/250 dilution, staining Rad17 in HeLa cells by immunofluorescence.
  • Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (ab247429)
    Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free (ab247429)

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