Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3968(2)] to TEF1/TEAD-1 - BSA and Azide free
  • Suitable for: ICC, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free
    See all TEF1/TEAD-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3968(2)] to TEF1/TEAD-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A549, HeLa, A431, 293T, and fetal muscle lysates; and HeLa cells.

  • General notes

    Ab248538 is the carrier-free version of ab133535. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248538 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)
    Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)
    All lanes : Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : TEAD1 knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab133535).

    Lanes 1 - 2: Merged signal (red and green). Green - ab133535 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab133535 was shown to react with TEF1/TEAD-1 in wild-type A549 cells in western blot with loss of signal observed in TEAD1 knockout sample. Wild-type and TEAD1 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab133535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)
    Immunocytochemistry - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)
    This data was developed using ab133535, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labelling TEF1/TEAD-1 with ab133535 at 1/50
  • Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)
    Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)
    All lanes : Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 4 : Fetal muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 48 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    This data was developed using ab133535, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)
    OI-RD Scanning - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)
    This data was developed using ab133535, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)
    Anti-TEF1/TEAD-1 antibody [EPR3968(2)] - BSA and Azide free (ab248538)

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