Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13227(B)] to MVP - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MVP antibody [EPR13227(B)] - BSA and Azide free
    See all MVP primary antibodies

  • Description

    Rabbit monoclonal [EPR13227(B)] to MVP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240184 is the carrier-free version of ab175239.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Western blot - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
    Western blot - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
    All lanes : Anti-MVP antibody [EPR13227(B)] (ab175239) at 1/2000 dilution

    Lane 1 : Wild-type HeLa lysate
    Lane 2 : MVP knockout HeLa lysate
    Lane 3 : A549 lysate
    Lane 4 : MOLT-4 lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 99 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab175239).

    Lanes 1-4: Merged signal (red and green). Green - ab175239 observed at 110 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab175239 Anti-MVP antibody [EPR13227(B)] was shown to specifically react with MVP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264817 (knockout cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. ab175239 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Flow Cytometry - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
    Flow Cytometry - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)

    Flow Cytometry analysis of A549 cells labelling MVP with purified ab175239 at a dilution of 1/180 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175239).

  • Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
    Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MVP with purified ab175239 at a dilution of 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/150) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175239).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling MVP with purified ab175239 at a dilution of 1/350. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175239).

  • Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
    Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)

    Immunocytochemistry/Immunofluorescence analysis of A549 cells labeling MVP with unpurified ab175239 at a dilution of 1/250 (red) and DAPI staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175239).

  • Flow Cytometry - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
    Flow Cytometry - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)

    Flow Cytometrical analysis of permeabilized A549 cells labeling MVP with unpurified ab175239 antibody at a dilution of 1/100 (red) compared to a negative control (Rabbit IgG, green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175239).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labeling MVP with unpurified ab175239 at a dilution of 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175239).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling MVP with unpurified ab175239 at a dilution of 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175239).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
    Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)

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