Anti-MVP antibody (ab226248)
Key features and details
- Rabbit polyclonal to MVP
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MVP antibody
See all MVP primary antibodies -
Description
Rabbit polyclonal to MVP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human MVP aa 843-893. The exact sequence is proprietary.
Database link: Q14764 -
Positive control
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226248 was affinity purified using an epitope specific to MVP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MVP antibody (ab226248)All lanes : Anti-MVP antibody (ab226248) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-MVP antibody (ab226248)MVP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226248 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226248 at 0.4 µg/ml.
Lane 1: ab226248 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody (ab226248)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for MVP using ab226248 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
