Anti-TCOF1 antibody (ab224544)
Key features and details
- Rabbit polyclonal to TCOF1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TCOF1 antibody
See all TCOF1 primary antibodies -
Description
Rabbit polyclonal to TCOF1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human TCOF1 aa 550-700.
Database link: Q13428 -
Positive control
- IHC-P: Human testis, Fallopian tube and rectum tissue; ICC: U-2 OS cells; WB: Caco-2 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (ab224544)
Immunohistochemical analysis of human testis tissue labeling TCOF1 in the nucleus and nucleoli of cells in seminiferous ducts with ab224544 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-TCOF1 antibody (ab224544)Immunofluorescent analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling TCOF1 (green) in the nucleoli fibrillar center with ab224544 at 2 µg/ml.
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Western blot - Anti-TCOF1 antibody (ab224544)Anti-TCOF1 antibody (ab224544) at 0.4 µg/ml + Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
Predicted band size: 152 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (ab224544)Immunohistochemical analysis of human Fallopia tube tissue labeling TCOF1 in the nucleus of glandular cells with ab224544 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (ab224544)Immunohistochemical analysis of human rectum tissue labeling TCOF1 in the nucleus and nucleoli of glandular cells with ab224544 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (ab224544)Immunohistochemical analysis of human liver tissue labeling TCOF1 (very low positivity) with ab224544 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
