ICC/IF image of ab111255 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111255, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96887, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa and HepG2 cells at 10µg/ml, and in 100% methanol fixed (5 min) Hek293 cells at 10µg/ml.

All lanes : Anti-BORIS antibody [KT72] (ab111255)

Lane 1 : BORIS with N-terminal fused tag.
Lane 2 : BORIS with C-terminal fused tag.

Predicted band size: 73 kDa



Tag is a proprietary ~26 kDa tag.