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Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)

Key features and details

  • Rat monoclonal [2H10] to NUP98 - Nuclear Pore Marker
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG2c

Overview

  • Product name

    Anti-NUP98 antibody [2H10] - Nuclear Pore Marker
    See all NUP98 primary antibodies

  • Description

    Rat monoclonal [2H10] to NUP98 - Nuclear Pore Marker

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    African green monkey
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human NUP98 aa 1-466.

  • Positive control

    • WB: HeLa nuclear lysate. Jurkat, HeLa, COS-7, NIH/3T3 and SH-SY5Y cell lysate. ICC/IF: HeLa and NIH/3T3 cells.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) Di Nunzio et al PLoS One. 2012;7(9):e46037. doi: 10.1371/journal.pone.0046037. Epub 2012 Sep 25. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Lentiviral vector-encoded shRNAs achieve efficient knock-down of human nucleoporins and have negligible cytotoxic or cytostatic effects.

    HeLa cells (4×106) were transduced with lentiviral vectors (MOI 50) encoding shRNAs specific for the indicated nucleoporins and used at 2 days p.t for Nup153 shRNA and 5 days p.t for all others.

    (Panel B) Subcellular localisation of nuclear pore components upon nucleoporin knock-down was tested by confocal fluorescence microscopy of LV- (Control) and LV-shRNA transduced cells using specific anti-Nup antibodies. Images were acquired on the same day with the same conditions and are representative of two independent experiments.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)
    Western blot - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)
    All lanes : Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) at 1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
    Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate
    Lane 6 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
    Lane 7 : P19 cell lysate
    Lane 8 : NRK cell lysate

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase

    Developed using the ECL technique.


    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) Image and protocol courtesy of Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome La Sapienza, Italy

    ab50610 (1/100) staining NUP98 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (green).

    Cells were fixed with paraformaldehyde/Triton X-100 [10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton X-100 at room temperature] or methanol (6 min in methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with DAPI in order to highlight the nucleus (blue).

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) Image and protocol courtesy of Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome La Sapienza, Italy

    ab50610 (1/100) staining NUP98 in NIH/3T3 (Mouse embryo fibroblast cell line) cells (green).

    Cells were fixed with paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton X-100 at room temperature) or methanol (6 min in methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with DAPI in order to highlight the nucleus (blue).

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) This image is courtesy of an anonymous Abreview.

    Paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for NUP98 (green) using ab50610 at 1/200 dilution in ICC/IF, followed by Donkey Anti-Rat Alexa Fluor® 488.

    This image was generated using the ascites version of the product.

    See Abreview

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