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Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)

Key features and details

  • Mouse monoclonal [21A10] to NUP98
  • Suitable for: WB, ICC/IF, Functional Studies
  • Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe
  • Isotype: IgG1

Overview

  • Product name

    Anti-NUP98 antibody [21A10] - BSA and Azide free
    See all NUP98 primary antibodies

  • Description

    Mouse monoclonal [21A10] to NUP98

  • Host species

    Mouse

  • Specificity

    ab179909 crossreacts with multiple nucleoproteins of S. cerevisiae, e.g. Nup116, Nup100, Nup145N, Nup57 and Nup9.

  • Tested applications

    Suitable for: WB, ICC/IF, Functional Studiesmore details

  • Species reactivity

    Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 1-29 (N terminal).
    Sequence:

    MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ


    Database link: D3KYQ3

    Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 646-664.
    Sequence: SNPTQGGGLFGAANPGLGG
    Database link: D3KYQ3
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    GLF

  • Positive control

    • Tetrahymena thermophila, S. pombe and S. cerevisiae cells and cell extracts. HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Western blot - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 0.4 µg/ml + HeLa cell extract

    Secondary
    HRP-labeled anti-mouse IgG at 0.4 µg/ml

    Developed using the ECL technique.

    Predicted band size: 112 kDa



    Image shows unsuitability of this antibody for Human samples.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)

    Immunofluorescence analysis of methanol-fixed HeLa cells, labeling NUP98 using ab179909 at 0.5 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 µg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged colored images.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)

    Immunofluorescence analysis of methanol-fixed Tetrahymena thermophila cells, labeling NUP98 using ab179909 at 0.5 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 µg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells. The open arrow indicates the micronucleus. Insets are magnified images showing the position of the micronucleus.

  • Western blot - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Western blot - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    All lanes : Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml

    All lanes : Tetrahymena thermophila cell extract

    Developed using the ECL technique.

    Predicted band size: 112 kDa
    Observed band size: 98 kDa
    why is the actual band size different from the predicted?



    Open arrowheads correspond to NUP98. Asterisk represents uncharacterized protein. For lane 2 exposure time was about 10 times longer than for lane 1.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)

    Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. pombe cells, labeling NUP98 using ab179909 at 10 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.

  • Western blot - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Western blot - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    All lanes : Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml

    Lane 1 : Cell extract from S. pombe wild type
    Lane 2 : Cell extract from S. pombe expressing endogenously NUP98 fused to a fluorescence protein

    Developed using the ECL technique.

    Predicted band size: 112 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)

    Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. cerevisiae cells, labeling NUP98 using ab179909 at 10 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.

  • Western blot - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Western blot - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml + S. cerevisiae cell extract

    Developed using the ECL technique.

    Predicted band size: 112 kDa

  • Functional Studies - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
    Functional Studies - Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)

    Summary of the suitability of ab179909 for immunological applications. IF: indirect immunofluorescence staining; WB: Western blotting analysis.

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