Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)
![Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)](https://www.abcam.com/ps/products/256/ab256552/Images/ab256552-353154-AntiNUP98-antibody-EPR22818128--BSA-and-Azide-free-western-blot-testis-kidney-mouse-human.jpg "Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22818-128] to NUP98 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free
See all NUP98 primary antibodies -
Description
Rabbit monoclonal [EPR22818-128] to NUP98 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, MCF7, HepG2, NIH/3T3, A20, PC-12, RAW264.7 whole cell lysate. Mouse testis and kidney tissue lysate. Human testis tissue lysate.
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General notes
Ab256552 is the carrier-free version of ab234434. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256552 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22818-128 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)All lanes : Anti-NUP98 antibody [EPR22818-128] (ab234434) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Observed band size: 98 kDa why is the actual band size different from the predicted?Exposure time: Lanes 1-2: 15 seconds Lane 3: 5.5 seconds.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234434).
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![Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)](https://www.abcam.com/ps/products/256/ab256552/Images/ab256552-2-benefits-of-recombinant-antibodies.png)
Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)
