Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NUP98 antibody [EPR6678] - BSA and Azide free (ab226102)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6678] to NUP98 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NUP98 antibody [EPR6678] - BSA and Azide free
    See all NUP98 primary antibodies

  • Description

    Rabbit monoclonal [EPR6678] to NUP98 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises Nuclear pore complex protein Nup96 of Nuclear pore complex protein Nup98-Nup96.

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, SH-SY5Y, and Caco-2 cell lysates and Human colon tissue.

  • General notes

    ab226102 is the carrier-free version of ab124980.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Western blot - Anti-NUP98 antibody [EPR6678] - BSA and Azide free (ab226102)
    Western blot - Anti-NUP98 antibody [EPR6678] - BSA and Azide free (ab226102)
    All lanes : Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 188 kDa



    This data was developed using ab124980, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-NUP98 antibody [EPR6678] - BSA and Azide free (ab226102)
    Immunocytochemistry - Anti-NUP98 antibody [EPR6678] - BSA and Azide free (ab226102)
    This data was developed using ab124980, the same antibody clone in a different buffer formulation.ab124980 staining NUP98 in SH-SY5Y, human neuroblastoma epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500). Ab150077, an Alexa Fluor® 594-conjugated Goat anti rabbit (1:1000) (ab150077) was used as the secondary antibody. Counterstained with ab195889, an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (1/100). Nuclear counterstained with DAPI.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP98 antibody [EPR6678] - BSA and Azide free (ab226102)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP98 antibody [EPR6678] - BSA and Azide free (ab226102)
    This data was developed using ab124980, the same antibody clone in a different buffer formulation.ab124980, at 1/50 to 1/100, staining NUP98 in Human colon tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)]. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-NUP98 antibody [EPR6678] - BSA and Azide free (ab226102)
    Anti-NUP98 antibody [EPR6678] - BSA and Azide free (ab226102)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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