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Anti-NUP98 antibody [2H10] - BSA and Azide free (ab252256)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [2H10] to NUP98 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NUP98 antibody [2H10] - BSA and Azide free
    See all NUP98 primary antibodies

  • Description

    Rat monoclonal [2H10] to NUP98 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: RAW264.7, HeLa, SH-SY5Y, PC-12 and NIH/3T3 whole cell lysate.

  • General notes

    ab252256 is the PBS only version of ab50610.

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

Properties

Images

  • Western blot - Anti-NUP98 antibody [2H10] - BSA and Azide free (ab252256)
    Western blot - Anti-NUP98 antibody [2H10] - BSA and Azide free (ab252256)
    All lanes : Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/50000 dilution

    Predicted band size: 198 kDa


    Exposure time: 3 minutes


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab50610).

    Blocking/Dilution buffer: 5% NFDM.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 17442773).

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-NUP98 antibody [2H10] - BSA and Azide free (ab252256)
    Western blot - Anti-NUP98 antibody [2H10] - BSA and Azide free (ab252256)
    All lanes : Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) at 1/1000 dilution

    Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/50000 dilution

    Predicted band size: 198 kDa


    Exposure time: 3 minutes


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab50610).

    Blocking/Dilution buffer: 5% NFDM.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 17442773).

  • Anti-NUP98 antibody [2H10] - BSA and Azide free (ab252256)
    Anti-NUP98 antibody [2H10] - BSA and Azide free (ab252256)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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