Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6678] to NUP98 - Nuclear Pore Marker
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker
    See all NUP98 primary antibodies

  • Description

    Rabbit monoclonal [EPR6678] to NUP98 - Nuclear Pore Marker

  • Host species

    Rabbit

  • Specificity

    ab124980 recognises Nuclear pore complex protein Nup96 of Nuclear pore complex protein Nup98-Nup96.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NUP98 aa 1600-1700. The exact sequence is proprietary. The immunogen sequence is within Nuclear pore complex protein Nup96 of the Nuclear pore complex protein Nup98-Nup96.
    Database link: P52948

  • Positive control

    • HeLa, SH-SY5Y, and Caco-2 cell lysates and Human colon tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)
    Western blot - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)
    All lanes : Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : SH-SY5Y cell lysates
    Lane 3 : Caco-2 cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 188 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)

    ab124980 staining NUP98 in SH-SY5Y, human neuroblastoma epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500). Ab150077, an Alexa Fluor® 594-conjugated Goat anti rabbit (1:1000) (ab150077) was used as the secondary antibody. Counterstained with ab195889, an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (1/100). Nuclear counterstained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)

    ab124980, at 1/50 to 1/100, staining NUP98 in Human colon tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)
    Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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