Anti-SARA antibody [EPR5020] - BSA and Azide free (ab248032)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5020] to SARA - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-SARA antibody [EPR5020] - BSA and Azide free
See all SARA primary antibodies -
Description
Rabbit monoclonal [EPR5020] to SARA - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248032 is the carrier-free version of ab124875 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248032 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 3.10 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5020 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution
Lane 1 : Human heart tissue lysate
Lane 2 : A673 cell lysate
Lane 3 : HT1080 cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 156 kDaThis data was developed using ab124875, the same antibody clone in a different buffer formulation.
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All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 156 kDaThis data was developed using ab124875, the same antibody clone in a different buffer formulation.
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This data was developed using ab124875, the same antibody clone in a different buffer formulation.Staining of SARA in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124875 at a dilution of 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab124875, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
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